Human Signal transducer and activator of transcription 3 (STAT3) ELISA Kit

Assay Genie offers this Sandwich ELISA for the quantitative detection of human Signal transducer and activator of transcription 3 (STAT3), also known as Acute-phase response factor (APRF). The kit is validated for use with human serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids, making it suited to a range of research applications including cancer research.

Performance specifications reported by the manufacturer include a detection range of 0.312–20 ng/mL and a sensitivity of 0.12 ng/mL. Assay precision is given as an intra-assay CV of 5.2% and an inter-assay CV of 9.4%. Spike-and-recovery values are reported as 88% (range 82–94%) for serum and 90% (range 84–96%) for plasma. Linearity has been assessed for serum, EDTA plasma and heparin plasma across dilution factors from 1:2 to 1:16.

STAT3 is a transcription factor of the STAT family that mediates cellular responses to interleukins and other growth factors, with dysregulation linked to cancer, inflammation and autoimmune disease. The kit is intended for research use only and is associated with UniProt entry P40763.