Human Signal Transducer And Activator Of Transcription 1 (STAT1) ELISA Kit

Quantitative measurement of Signal Transducer and Activator of Transcription 1 (STAT1) in human samples is the focus of this Assay Genie ELISA kit, which gives researchers a robust tool to investigate interferon signaling and downstream immune responses. STAT1 is a key cytoplasmic transcription factor that becomes phosphorylated in response to type I (IFN-alpha and IFN-beta) and type II (IFN-gamma) interferons, then dimerizes and translocates to the nucleus to drive expression of interferon-stimulated genes involved in antiviral defense, cell proliferation, and apoptosis.

The kit is configured for 96 assays and validated for tissue homogenates and cell lysates. Researchers studying JAK-STAT pathway dysregulation in cancer, autoimmune disease, or chronic inflammation can monitor STAT1 protein levels across experimental models. The colorimetric readout is measured on standard microplate readers, and the kit is supplied with all reagents required to generate a standard curve and run samples in duplicate.

Each kit includes a pre-coated microplate, lyophilized standards, detection antibody, dilution buffers, wash buffer, substrate solution, and stop solution. The protocol follows a standard workflow and is intended for research use only. STAT1 quantification can support studies of drug response, gene knockout effects, and disease mechanisms in which interferon signaling is implicated.