Human Signal Regulatory Protein Gamma (SIRPg) ELISA Kit

Signal Regulatory Protein Gamma (SIRPg) is a transmembrane glycoprotein belonging to the immunoglobulin superfamily, expressed on T lymphocytes, NK cells, and select myeloid populations where it engages CD47 on neighboring cells. This Assay Genie kit delivers a quantitative solution for measuring human SIRPg in tissue homogenates and cell lysates, supporting immunology, oncology, and cell-signaling research programs.

The assay uses a 96-assay plate layout with a colorimetric readout compatible with standard microplate readers. Researchers can apply the kit to profile SIRPg expression in tumor-infiltrating lymphocytes, characterize immune cell interactions in vitro, or track changes in cell-surface protein shedding during T-cell activation. The protocol is intended for research use only and supplies pre-coated wells, a biotinylated detection antibody, recombinant calibrators, streptavidin-HRP conjugate, and buffer reagents sufficient for 96 individual determinations.

Reagents should be handled according to the enclosed product insert, with working dilutions prepared fresh before each run and conjugate components protected from light. The kit is validated by Assay Genie for specificity to the human SIRPg extracellular domain, helping distinguish genuine target signal from background across heterogeneous sample types.