Human Siglec-6 PharmaGenie ELISA Kit (SBRS1092)

The Assay Genie Human Siglec-6 (CD327) PharmaGenie ELISA Kit enables quantitative measurement of soluble Siglec-6 across a panel of human biofluids, including cell culture supernatants, citrated plasma, and serum samples, using a colorimetric microplate readout. Siglec-6 is a sialic acid-binding immunoglobulin-type lectin expressed on B lymphocytes, mast cells, and placental trophoblasts, and serves as a biomarker in pregnancy biology, mast cell signalling studies, and B-cell immunophenotyping workflows. The kit uses a pre-coated microplate, biotinylated detection antibody, and streptavidin-HRP conjugate to generate signal proportional to bound analyte, with up to 96 individual determinations per plate. Reagents should be stored refrigerated; standard and sample aliquots must be kept frozen to limit degradation through repeated freeze-thaw cycles. The capture antibody is human-specific with no significant cross-reactivity with rat Siglec-F or mouse Siglec-F homologues, making it appropriate for in vitro research on human immune and reproductive biology. Each pack includes a detailed protocol with recommended dilution factors, incubation times, and wash steps to generate a standard curve for interpolating unknown Siglec-6 concentrations.