Human SIAH2 ELISA Kit (HUFI06523)

For quantitative analysis of SIAH2, an E3 ubiquitin-protein ligase central to hypoxia signaling and tumor biology, Assay Genie provides this ELISA-based research tool. Each kit supplies all reagents needed to perform 96 individual determinations on a standard microplate, with refrigerated storage recommended to preserve antibody and standard stability. SIAH2 has emerged as a research focus in melanoma, breast cancer, and neurodegenerative disease studies because of its role in targeting prolyl hydroxylases (PHD1/EGLN2 and PHD3/EGLN3), p53, and TRAF family proteins for proteasomal degradation. The assay accommodates serum specimens and plasma prepared from human whole blood and follows a standard laboratory workflow using a plate reader for absorbance measurement. Researchers exploring HIF-1α pathway regulation, mitochondrial apoptosis, or E3 ligase substrate specificity can integrate this kit into mechanistic and translational investigations. The package includes a pre-coated plate, detection antibody, standards, and buffers in sealed pouches, supporting in vitro research applications only. The protocol can be completed in a standard laboratory setting without specialized equipment beyond a microplate reader capable of measuring absorbance in the visible range.