Human SHPRH (E3 ubiquitin-protein ligase SHPRH) ELISA KIT (HUFI04871)

Quantitative measurement of SHPRH in research samples supports studies of DNA damage response, post-replication repair, and the ubiquitin-proteasome system. SHPRH encodes an E3 ubiquitin-protein ligase with RING, helicase, and PHD domains that polyubiquitinates PCNA at stalled replication forks, directing lesion bypass through the error-free pathway. Altered SHPRH expression has been reported in melanoma, breast, and other solid tumour investigations, making reliable quantification relevant to oncology and genome stability research.

The kit from Assay Genie is supplied in a 96 Assays format and uses an ELISA-based method. Researchers should refrigerate the kit on arrival and equilibrate reagents to room temperature before use. Reconstituted standards and prepared samples should be handled according to the enclosed protocol to preserve antigen integrity across freeze-thaw cycles.

SHPRH is a large, multidomain nuclear protein, and assays targeting it typically benefit from well-validated capture and detection antibodies. The kit is intended for research use only, supporting workflows that probe chromatin-associated ubiquitination, replication stress responses, and downstream pathway analysis in cell culture models and biological specimens.