Human Short Palate, Lung And Nasal Epithelium Carcinoma Associated Protein 3 (SPLUNC3) ELISA Kit

The SPLUNC3 protein, also known as BPIFB3 or LPLUNC3, belongs to the BPI/LBP/PLUNC superfamily of secreted mucosal proteins implicated in innate airway defense. This Assay Genie immunoassay is built on a 96-well microplate and uses a biotinylated capture antibody plus streptavidin-HRP signal amplification to quantify human SPLUNC3 in tissue homogenates. The kit includes a pre-coated plate, lyophilized recombinant standards, biotinylated detection reagent, streptavidin-HRP conjugate, wash buffer, and substrate, enough for one full plate run. Reconstituted standards should be aliquoted and frozen; the sealed plate should be kept refrigerated and protected from light until use. Researchers studying upper respiratory biology apply the test to characterize SPLUNC3 expression in lung and nasal epithelium samples, using a sample volume of 50 µL of clarified lysate per well over an approximately 3.5 hour protocol.