Human SHISA4 ELISA Kit (HUFI06531)

Built for researchers studying Wnt pathway modulation and ER-associated signaling, this quantitative immunoassay from Assay Genie measures human SHISA4, a single-pass transmembrane regulator of the Wnt/β-catenin cascade. SHISA4, also referenced as TMEM58 in earlier literature, localizes to the endoplasmic reticulum membrane and influences cell-fate decisions during development and in oncology contexts where Wnt signaling is dysregulated.

The kit is configured for use with a microplate reader workflow and accommodates standard biological sample preparation. Researchers commonly pair it with Western blot or qPCR validation to confirm transcript- and protein-level changes in SHISA4 expression across treated versus control samples.

Each kit ships with a pre-coated plate, detection reagents, standards, and the buffers required for the assay. The plate layout supports duplicate or triplicate determinations, allowing direct comparison of biological replicates within the same run. All components are intended for research use only and should be stored refrigerated to preserve reagent activity until first use.