Human SHCBP1 (SHC SH2 domain-binding protein 1) ELISA KIT (HUFI04885)

The Assay Genie SHCBP1 immunoassay quantifies SHC SH2 domain-binding protein 1, an adaptor protein that participates in SHC-mediated signaling pathways and mitotic cytokinesis. SHCBP1 localizes to the central spindle during cell division and supports proper cleavage furrow formation, with documented overexpression in hepatocellular carcinoma, gastric cancer, and glioma correlating with aggressive disease phenotypes and reduced survival.

This plate-based assay is intended for research use only and is configured for quantitative measurement in human samples. Investigators in oncology, cell biology, and signal transduction use this kit to evaluate SHCBP1 abundance across patient tissue cohorts, compare treated versus untreated cell lines, or screen for protein-level changes following siRNA or CRISPR knockdown experiments. The assay is also useful for monitoring SHCBP1 dynamics through the cell cycle and for profiling protein expression in tumor xenograft lysates.

Each kit ships with a pre-coated microplate, lyophilized recombinant protein standard, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer concentrate, and TMB substrate. Unopened kits should be refrigerated and the chromogen protected from light to preserve reagent activity. The supplied reagents support a full standard curve alongside unknown determinations, and each kit is supplied as a single-use pack with all buffers and diluents included for plate processing.