Human SHBG/Sex Hormone Binding Globulin ELISA Kit

A sandwich ELISA designed by Assay Genie for the quantitative measurement of human SHBG (Sex Hormone Binding Globulin), a glycoprotein that binds sex hormones such as testosterone and estradiol and plays a central role in maintaining hormonal balance. The kit is configured for use with human samples, including serum, plasma, cell culture supernatant, cell or tissue lysates, and other liquid samples. Detection is colorimetric, the assay type is a double-antibody sandwich ELISA, and the kit should be stored at 2–8 °C for up to 12 months.

Performance characteristics reported for this Assay Genie kit include a sensitivity of 9.375 pmol/L and a working range of 15.625–1000 pmol/L. Linearity in serum (n = 5) spans 86–96% at 1:2, 85–95% at 1:4, and 82–99% at 1:8; EDTA plasma (n = 5) spans 85–104%, 82–101%, and 88–99% across the same dilutions; heparin plasma (n = 5) spans 87–105%, 87–100%, and 80–98%. Spike-and-recovery values are 89–97% (average 92%) for serum, 87–105% (average 98%) for EDTA plasma, and 86–100% (average 95%) for heparin plasma. The protocol uses a biotin-labelled detection antibody with HRP-Streptavidin (SABC) and TMB substrate, with absorbance read at 450 nm after stop solution addition.

Because the catalogue entry names this kit specifically as the "Human SHBG/Sex Hormone Binding Globulin ELISA Kit" and the description states it is "to measure SHBG/Sex Hormone Binding Globulin in serum samples," the same product name from Assay Genie is referenced; no pack size, part number, or catalogue number is stated in this description. Cross-reactivity data and intra-/inter-assay precision values are not stated in the labelled sources for this kit and are therefore omitted rather than supplied.