Human SH3YL1 (SH3 domain-containing YSC84-like protein 1) ELISA Kit (HUES03601)

SH3YL1 encodes an SH3 domain-containing protein implicated in phosphoinositide metabolism, actin cytoskeleton remodeling, and membrane trafficking at the cell periphery. The kit from Assay Genie uses a sandwich ELISA configuration with colorimetric detection, providing quantitative measurement of human SH3YL1 in serum, plasma, and other biological fluids. Each kit contains reagents sufficient for 96 assays on a microplate, with results readable on a standard plate reader at 450 nm. Reconstituted standards and biotinylated detection antibody should be stored at -20 °C and protected from light, while the microplate and other components are kept refrigerated at 2-8 °C; repeated freeze-thaw cycles of the reconstituted reagents should be avoided. The assay is validated for research use only, supporting investigations into cell migration, endocytic pathways, and tumor biology, where altered SH3YL1 expression has been linked to non-small cell lung carcinoma progression and other malignancies. Typical sample volumes of 100 µl per well yield a standard curve spanning low pg/ml to upper ng/ml concentrations. Investigators in oncology, cell signaling, and membrane biology can apply this ELISA to quantify SH3YL1 levels across patient cohorts, knockout models, or in vitro stimulation experiments probing phosphoinositide-dependent signaling cascades.