Human SH3PXD2A ELISA Kit (HUFI06537)

SH3PXD2A, also known as TKS5 or FISH (Five SH3 domains), is a large scaffolding adaptor containing five Src-homology 3 domains and a single PX domain that docks to phosphatidylinositol 3,4,5-trisphosphate-rich membranes. It is a direct substrate of Src family kinases, phosphorylated on tyrosine residues during integrin and growth factor signalling, and is the defining molecular marker for mature invadopodia and podosomes, the ventral actin protrusions used by cancer cells and osteoclasts to locally degrade the extracellular matrix.

This Assay Genie quantitative immunoassay uses a human-specific capture antibody pair for measurement of full-length SH3PXD2A from circulating blood fractions and cultured cell lysate preparations. Each 96 Assays kit ships with a pre-coated microplate, biotinylated detection reagent, streptavidin-HRP conjugate, chromogenic substrate, stop reagent, wash concentrate, and calibrated recombinant standards. Recommended sample volume is 100 µl per well, and unused strips should be resealed in the provided pouch with desiccant.

Store the sealed kit refrigerated at 4 degrees C and bring all components to room temperature before each run. The substrate vial should be protected from light, and reconstituted standards aliquoted and frozen for long-term stability. For research use only, supporting studies in tumour cell invasion, Src-pathway signalling, podosome biology in macrophages and osteoclasts, and biomarker work in breast carcinoma, melanoma, and head and neck squamous cell carcinoma.