Human SGK1 PharmaGenie ELISA Kit (SBRS1084)

Researchers tracking Serum and Glucocorticoid-regulated Kinase 1 (SGK1) in clinical, cellular, and translational contexts rely on the Assay Genie ELISA kit for quantitative protein-level readouts. SGK1 is a serine/threonine kinase activated downstream of glucocorticoid, mineralocorticoid, and PI3K-PDK1 signaling, with reported substrates including NDRG1, FOXO3 transcription factors, and ENaC sodium channel subunits. The microplate format accommodates standards alongside unknown human specimens processed in parallel — including matched serum draws, plasma aliquots, and cell culture supernatant fractions — supporting direct comparison across treatment arms. Use this kit to corroborate Western blot quantification of phosphorylated SGK1, validate SGK1 transcript changes measured by real-time PCR, or profile kinase response in renal tubular, hepatic, and neuronal model systems. Bring all working reagents to room temperature prior to plate loading; reconstituted standard vials and detection antibody are aliquoted and stored frozen to limit freeze-thaw exposure, while labelled conjugates are kept protected from light. The kit ships refrigerated in 96-assay packaging and is intended for research use only.