Human SFRP2/Secreted frizzled-related protein 2 ELISA Kit

Manufactured by Assay Genie, this sandwich ELISA kit is designed for the quantitative measurement of human Secreted Frizzled-Related Protein 2 (SFRP2), a modulator of the Wnt signaling pathway that influences cell growth, differentiation, and tissue development. The colorimetric assay is validated for use with human serum, plasma, cell culture supernatant, cell or tissue lysates, and other liquid samples, with a reported sensitivity of 0.75 ng/mL and a working range of 1.25-80 ng/mL. Reagents are stored at 2-8°C for 12 months.

The protocol proceeds through reagent equilibration, a primary incubation at 37°C for 90 minutes, biotinylated detection antibody binding, HRP-streptavidin (SABC) binding, TMB color development, and a stop reaction read at 450 nm. Reported linearity for serum (n=5) spans 81-100% across 1:2 to 1:8 dilutions, 82-104% for EDTA plasma (n=5), and 85-105% for heparin plasma (n=5). Spike recovery averages 91% for serum (n=5), 96% for EDTA plasma (n=5), and 95% for heparin plasma (n=5).

The kit supports research into SFRP2 dysregulation, which has been implicated in cancer, fibrosis, and cardiovascular disorders, and is intended for studies of disease pathogenesis, biomarker discovery, and drug development.