Human SF3A1 ELISA Kit (HUFI06580)

SF3A1 (Splicing Factor 3a Subunit 1) is a 120 kDa scaffolding component of the U2 small nuclear ribonucleoprotein, anchoring the spliceosome to the branch-point sequence during pre-mRNA splicing. Mutations and altered expression of SF3A1 have been associated with myelodysplastic syndromes, chronic lymphocytic leukemia, and several solid tumors, making quantitative measurement of this splicing factor in clinical research samples of growing interest.

The Assay Genie Human SF3A1 ELISA Kit provides 96 individual determinations through a quantitative enzyme-linked immunosorbent assay formatted for in vitro research use only. Optimized for prepared human serum-derived and plasma-derived specimens, the workflow applies an HRP-driven chromogenic readout to convert bound SF3A1 into a quantifiable signal, supporting biomarker discovery, spliceosome-function studies, and RNA-processing investigations.

For reproducible results, store unopened components at refrigerated temperatures, avoid freezing the pre-coated microplate, protect the streptavidin-HRP conjugate from light, and equilibrate all reagents to room temperature before each run. Each package ships with a pre-coated microplate, lyophilized reference standards, biotinylated detection antibody, streptavidin-HRP, wash buffer, and chromogenic substrate sufficient to complete the full 96-assay workflow.