Human Sex hormone-binding globulin (SHBG) ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA kit is designed for the quantitative measurement of Sex hormone-binding globulin (SHBG) in human samples. SHBG functions as an androgen transport protein, with each dimer binding one molecule of steroid; it is specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol, and regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration. The kit is intended for research use only.

The assay delivers a detection range of 7.8-500nmol/L with a sensitivity of 3.9nmol/L. Precision is reported with an intra-assay CV of 4.1% and an inter-assay CV of 7.1%. Acceptable sample types include serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids, with specificity demonstrated for natural and recombinant human SHBG. Recovery in serum averages 103% (range 97-109%) and in plasma averages 105% (range 99-111%). Recognised synonyms include Sex steroid-binding protein, SBP, ABP, TeBG, Testis-specific androgen-binding protein, and Testosterone-estradiol-binding globulin.

The homodimeric protein has a molecular weight of 43,779 Da (UniProt P04278) and is listed under the cardiovascular research area. It is secreted into the extracellular region and, in testis, is synthesised by Sertoli cells, secreted into the lumen of the seminiferous tubule, and transported to the epididymis.