Human Sex Hormone Binding Globulin (SHBG) ELISA Kit

SHBG (Sex Hormone Binding Globulin) is a hepatic glycoprotein produced by the liver that binds circulating androgens and estrogens, regulating the bioavailability of testosterone and estradiol in circulation. Quantification of SHBG supports clinical evaluation of androgen excess, polycystic ovary syndrome, hirsutism, infertility workups, thyroid disorders, and metabolic syndrome, and enables calculation of the free androgen index. This Assay Genie kit is supplied as a complete set of pre-coated microplate wells, lyophilized reference standards, biotinylated detection antibody, wash concentrate, substrate solution, and stop reagent. The protocol produces a quantifiable calibration curve across the working range for accurate concentration determination from a 50 uL sample volume per well. Researchers in endocrinology, reproductive biology, and metabolic studies apply this ELISA in vitro to investigate steroid hormone transport and assess hepatic, thyroid, and gonadal function.