Human Sestrin 2 (SESN2) ELISA Kit

Quantitative measurement of SESN2 protein in human specimens is supported by the Assay Genie Human Sestrin 2 (SESN2) ELISA Kit, enabling research into cellular stress-response pathways. Sestrin 2 acts as a stress-inducible regulator linking oxidative challenge to mTORC1 inhibition and autophagy induction, and accurate quantification supports investigations of metabolic dysfunction, neurodegeneration, and tumor progression.

Built for analysis of tissue homogenates and assorted human-derived sample matrices, this 96-assay kit accommodates multiple specimens per plate, providing flexibility for both small pilot studies and larger screening efforts. Researchers commonly apply it to lysates from cultured cell lines exposed to oxidative, genotoxic, or nutrient stress conditions, and to tissue extracts probing disease-associated expression changes.

The kit contains pre-coated microwell strips, biotinylated detection antibody, reconstituted standard, streptavidin-HRP conjugate, substrate solution, wash buffer, and stop reagent. Color development is measured spectrophotometrically, with absorbance readings converted to concentration values using the supplied standard curve. Standard curve fitting follows a four-parameter logistic model for accurate interpolation across the working range.

Short-term working aliquots of prepared standards and samples should be kept on ice, with the kit components themselves stored under refrigerated conditions between uses to preserve reagent activity. Repeated freeze-thaw cycles of reconstituted standards should be avoided to maintain calibration integrity.