Human Serum Amyloid A2/SAA2 ELISA Kit

Designed by Assay Genie, this sandwich ELISA kit enables precise quantification of Serum Amyloid A2 (SAA2) levels in human research samples. The kit uses a double-antibody, colorimetric detection method with a sensitivity of 0.938 ng/mL and a working range of 1.563-100 ng/mL, making it suitable for measuring the acute-phase inflammatory protein SAA2 across a range of study designs.

SAA2 is recognised as a valuable biomarker for conditions such as cardiovascular diseases, diabetes, and rheumatoid arthritis, supporting research into inflammation-driven disease mechanisms and potential therapeutic targets. Compatible sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit is stored at 2-8°C for up to 12 months and follows a six-step protocol: reagent equilibration and plate setup, primary incubation at 37°C for 90 minutes, biotin-labelled detection antibody incubation for 60 minutes, HRP-Streptavidin binding for 30 minutes, TMB colour development in the dark for 10-20 minutes, and absorbance reading at 450 nm after stop solution addition.

Validation data demonstrate strong linearity across serum, EDTA plasma, and heparin plasma at dilutions of 1:2, 1:4, and 1:8, with recovery rates typically between 85% and 105% depending on the matrix. Researchers in immunology and inflammation-related fields can rely on this kit for consistent, reproducible SAA2 measurement in basic and translational investigations.