Human Serpin A4 PharmaGenie ELISA Kit (SBRS1074)

The Assay Genie Human Serpin A4 ELISA Kit provides quantitative measurement of kallistatin (Serpin A4), a serine proteinase inhibitor implicated in vascular biology, inflammation regulation, and tissue remodeling research. The PharmaGenie platform supports translational workflows in cardiovascular, metabolic, and oncology studies where kallistatin levels serve as a biomarker of interest. Researchers apply the kit to investigate nitric oxide signaling, angiogenesis modulation, and inflammatory cascades using cell-derived samples and clinical study specimens. The microplate-based workflow, performed with a 50 µL sample volume per well, includes pre-coated wells, lyophilized recombinant reference standards, biotinylated detection antibody, streptavidin-HRP conjugate, chromogenic substrate, wash buffer concentrate, and a detailed protocol insert. Standard curve generation with seven serial dilution points enables accurate interpolation, and intra-assay precision delivers reproducible quantification across drug discovery screening campaigns and pharmacokinetic studies.