Human Serpin A4/Kallistatin ELISA Kit

Built around a quantitative double-antibody sandwich immunoassay, this Assay Genie kit targets human Serpin A4/Kallistatin (SERPINA4, UniProt P29622), the secreted serine proteinase inhibitor best known for opposing tissue kallikrein activity and for its roles in vascular tone regulation, anti-angiogenic signaling, and modulation of inflammatory cascades. It is used in research on sepsis pathophysiology, hepatocellular carcinoma progression, diabetic complications, and wound healing, where serum and plasma Kallistatin concentrations serve as a window onto endothelial and inflammatory status.

The kit ships as a complete workflow: pre-coated 96-well strip microplate, lyophilized standard, biotinylated detection antibody, streptavidin-HRP conjugate, TMB one-step substrate, stop solution, wash buffer, and assay diluent, plus a printed protocol. Sample handling guidance specifies serum or plasma preparation, and the standard protocol uses 100 uL sample additions per well, with detection read at 450 nm on a standard microplate reader.

For storage and stability, unopened components should be kept refrigerated and the kit is supplied with a 6-month shelf life; reagents remain stable through the labeled expiration date when handled per the enclosed protocol and protected from repeated freeze-thaw cycles. The product is labeled for research use only and is intended for laboratory personnel running in vitro immunoassays on human specimens.