Human Serine/threonine-protein kinase/endoribonuclease IRE1 (ERN1) ELISA Kit

A sandwich ELISA kit from Assay Genie designed for the quantitative measurement of human serine/threonine-protein kinase/endoribonuclease IRE1 (ERN1), also known by the synonyms IRE1, IRE1a, hIRE1p, Ire1-alpha, Inositol-requiring protein 1, and Endoplasmic reticulum-to-nucleus signaling 1. ERN1 is a transmembrane protein kinase that functions as a sensor of unfolded proteins in the endoplasmic reticulum, triggering the unfolded protein response (UPR), an ER stress response conserved from yeast to mammals that activates genes involved in degrading misfolded proteins, regulating protein synthesis, and activating molecular chaperones.

The kit is validated for use with human serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids, and uses a colorimetric detection method. Performance characteristics include a detection range of 0.312-20 ng/mL, a sensitivity of 0.12 ng/mL, an intra-assay CV of 4.8%, and an inter-assay CV of 8.9%. Linearity and recovery have been evaluated in serum, EDTA plasma, and heparin plasma samples. The observed molecular weight of the target protein is 110 kDa. The kit is intended for signal transduction research and is for research use only.

ERN1 contains both a serine/threonine-protein kinase domain and an endoribonuclease domain. Upon sensing unfolded proteins in the ER lumen via its N-terminal domain, the protein undergoes auto-activation and its endoribonuclease domain splices XBP1 mRNA to generate a potent unfolded-protein response transcriptional activator, leading to growth arrest and apoptosis. Dysregulation of ERN1 has been linked to cancer, diabetes, and inflammatory disorders, making it a target of interest for therapeutic research. Specificity is reported for natural and recombinant human ERN1.