Human Serine/Threonine Kinase 39 (STK39) ELISA Kit

The Assay Genie STK39 ELISA kit is configured for quantitative measurement of human Serine/Threonine Kinase 39, the SPAK-family kinase that phosphorylates and activates NKCC1, NKCC2, and the renal sodium-chloride cotransporter NCC downstream of WNK kinases. Investigators working on salt-sensitive hypertension, renal tubule physiology, and cardiovascular signaling use this immunoassay to profile STK39 expression in tissue homogenates prepared from human kidney, vasculature, and neural sources. The colorimetric protocol, run on a 96-assay microplate, supports batched comparison of patient cohorts, knockout models, and pharmacological interventions targeting the WNK-SPAK-OSR1 pathway that governs ion homeostasis and blood pressure. Reagents and standards should be stored refrigerated and protected from light to preserve antibody binding activity across the standard curve, and the assay is intended for research use only. STK39 has emerged as a candidate gene in essential hypertension, making the kit applicable to genetic association studies, biomarker discovery projects, and mechanistic work on cellular volume regulation, with relevance to nephrology, cardiology, and signal transduction laboratories.