Human Septin 9 (Septin 9) ELISA Kit

Designed for the quantitative measurement of Septin 9 in human samples, this sandwich ELISA from Assay Genie uses a colorimetric detection method and a double antibody approach to deliver precise results. The kit is intended for research applications exploring Septin 9 biology, a protein associated with cell division and cytoskeletal organization whose abnormal levels have been linked to cancer, neurological disorders, and developmental anomalies.

Key performance specifications include a sensitivity of 0.188 ng/mL and a working range of 0.313–20 ng/mL. The assay is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Reagents are supplied for storage at 2-8°C, where the kit remains stable for 12 months. A six-step protocol guides users from plate preparation through primary incubation (90 minutes at 37°C), biotinylated detection antibody binding (60 minutes), HRP-streptavidin binding (30 minutes), TMB color development (10–20 minutes in the dark), and a final absorbance reading at 450 nm.