Human SENP1 ELISA Kit (HUFI06601)

Quantitative measurement of Sentrin-specific protease 1 in human research samples is enabled by this Assay Genie ELISA kit, which supports investigation of SUMOylation and deSUMOylation pathways central to post-translational modification biology. SENP1 is a nuclear cysteine protease that removes SUMO1 conjugates from substrate proteins, and altered expression has been linked to hypoxia signaling through HIF-1alpha stabilization, androgen receptor transcriptional activity, and proliferation pathways studied in prostate and hepatic tumor models.

Configured as a 96 Assays kit intended for research use only, the reagents are packaged in individual vials together with a capture plate. The workflow generates a measurable signal on a standard laboratory microplate reader, and standard curve materials are included for interpolating unknown sample concentrations. This format integrates into cell culture supernatant analysis and validation workflows alongside western blot and PCR confirmation of SENP1 transcript and protein changes.

Investigators studying SUMO pathway enzymes, hypoxic response regulation, or steroid receptor signaling will find this kit useful for tracking SENP1 protein abundance across experimental conditions. Reagents should be kept refrigerated until first use, with reconstituted standards handled according to the enclosed protocol to preserve activity across multiple freeze-thaw cycles.