Human Semaphorin-3F (SEMA3F) ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA kit is designed for the precise measurement of Human Semaphorin-3F (SEMA3F) in human samples including serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. The kit shows reactivity with human samples and detects natural and recombinant human Semaphorin-3F. SEMA3F is a member of the semaphorin III family of secreted signalling proteins involved in axon guidance during neuronal development, and may play a role in cell motility and cell adhesion. Dysregulation of SEMA3F has been implicated in various diseases including cancer, autoimmune disorders and neurological conditions, making it a valuable biomarker for research.

The target protein is also known as Sema III/F, Semaphorin IV or Sema IV. SEMA3F is a secreted protein with a molecular weight of 88 kDa, encoded at chromosomal location 3p21.31 with UniProt accession Q13275. The research area is Neurosciences.

Performance characteristics include a detection range of 0.156-10 ng/mL, sensitivity of 0.042 ng/mL, intra-assay CV of 5.3% and inter-assay CV of 8.3%. Linearity in serum samples ranges from 98-107% at 1:2 dilution to 90-103% at 1:16, while recovery in serum averages 101% (range 95-107%).