Human Semaphorin 3A (SEMA3A) ELISA Kit

Secreted by cells in the nervous and immune systems, Semaphorin 3A (SEMA3A) acts through neuropilin-1 and plexin-A receptors to direct axon guidance, modulate regulatory T cell function, and shape tumor microenvironments. This Assay Genie ELISA kit enables quantitative measurement of Human SEMA3A across research specimens relevant to neuroscience, immunology, and oncology laboratories.

Validated sample matrices include tissue homogenates and cell culture supernatants. It provides a microplate with pre-coated wells, biotinylated detection antibody, standard diluent, wash buffer, and substrate solution, configured to run up to 96 determinations per microplate. A standard curve is generated from serial dilutions of the provided recombinant SEMA3A reference, allowing interpolation of unknown sample concentrations.

Reagents should be stored refrigerated between uses to preserve activity through the stated shelf life, and the kit is validated for research use only in laboratory settings. Typical research applications extend to multiple sclerosis and Alzheimer's biomarker studies, vascular biology investigations, autoimmune disease research, and cardiac remodeling studies, where circulating SEMA3A levels serve as a measurable indicator of pathway activity in in vitro experimental workflows.