Human SEMA5A/Semaphorin-5A ELISA Kit

Assay Genie supplies this Sandwich ELISA, Double Antibody kit for the precise quantification of SEMA5A levels in human samples, supporting research into nervous system development and function. The kit is intended for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Detection is colorimetric, with a sensitivity of 0.188 ng/mL and a working range of 0.313-20 ng/mL. Kit components are stored at 2-8°C for 12 months.

Reported linearity covers serum (88-104% / 87-105% / 91-102% at 1:2, 1:4, 1:8 dilutions), EDTA plasma (85-100% / 84-95% / 83-101%), and heparin plasma (87-100% / 82-94% / 81-86%). Recovery ranges from 85-101% (average 93%) in serum, 88-100% (average 95%) in EDTA plasma, and 88-105% (average 95%) in heparin plasma. SEMA5A is implicated in axon guidance and cell migration, and its dysregulation has been linked to neurological disorders including autism and schizophrenia.