Human SEMA3D ELISA Kit (HUFI06611)

The Human SEMA3D ELISA Kit from Assay Genie provides researchers with a quantitative immunoassay for measuring Semaphorin 3D, a secreted signaling protein implicated in axon guidance, vascular patterning, and bone metabolism. SEMA3D belongs to the class 3 semaphorin family and has been investigated in oncology, neurobiology, and skeletal research, where its circulating concentrations are of interest as a potential biomarker.

This assay uses pre-coated microplate wells, calibrated recombinant standards, and a horseradish peroxidase detection system to deliver reproducible measurements. Standards and unknown samples are added to wells, followed by a biotinylated detection antibody and streptavidin-HRP conjugate, with absorbance readings taken after TMB substrate addition. Each kit supports analysis of unknowns alongside a standard curve and includes all buffers and reagents necessary for the complete workflow.

Allow all kit components to reach ambient temperature before use, and store the kit refrigerated between sessions. Reconstituted standards and concentrated reagents should be aliquoted to limit repeated freeze-thaw cycles. The kit is supplied with a comprehensive protocol booklet outlining plate layout, dilution schemes, and recommended quality-control practices.