Human SEMA3A (Semaphorin 3A) CLIA Kit (HUES00730)

A chemiluminescence ELISA kit from Assay Genie for the in vitro quantitative determination of Human SEMA3A (Semaphorin 3A). The assay uses a Sandwich-CLIA format in which the microplate is pre-coated with a Human SEMA3A-specific antibody. Standards or samples are added to the coated wells, followed by a biotinylated detection antibody and an Avidin-Horseradish Peroxidase (HRP) conjugate. After washing, a substrate solution is added and the resulting Relative Light Unit (RLU) signal is read on a chemiluminescence immunoassay analyzer; RLU values are positively associated with the concentration of Human SEMA3A.

According to the manufacturer, the kit has a sensitivity of 18.75 pg/mL and a detection range of 31.25-2000 pg/mL, with an assay time of approximately 3.5 hours. It is intended for use with serum, plasma and other biological fluids. The manufacturer states that the kit recognizes Human SEMA3A in samples and that no significant cross-reactivity or interference between Human SEMA3A and analogues was observed. Storage conditions listed in the datasheet include -20°C for the coated plate, reference standard and concentrated biotinylated detection antibody and HRP conjugate, and 4°C for the reference standard and sample diluent as well as the substrate reagents.

Third-party supplier data describes the format as a solid-phase CLIA kit reactive with human samples, shipped at +4°C. Safety information specifies the signal word "Warning" with hazard statements H315 (skin irritation) and H319 (eye irritation) and precautionary statements P264 (wash hands thoroughly after handling) and P280 (wear protective gloves/clothing and eye/face protection). The product is associated with KEGG entry K06840, UniProt ID Q14563 and Gene ID 10371. Assay Genie positions the kit for research in Developmental Biology and Human Immunology, describing SEMA3A as a signaling protein involved in neuronal development, immune regulation and cancer progression.