Human Secreted frizzled-related protein 2 (SFRP2) ELISA Kit

From Assay Genie, this Sandwich ELISA is designed for the accurate detection of Human Secreted frizzled-related protein 2 (SFRP2) levels in human serum, plasma, and cell culture supernatants, with stated high sensitivity and specificity for reliable, reproducible results across a range of research applications.

The kit employs a colorimetric detection method and is reactive with human samples. Compatible sample types include serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, with specificity directed at natural and recombinant human Secreted frizzled-related protein 2. Quoted performance figures include a detection range of 1.25-80ng/mL and a sensitivity of 0.65ng/mL. Reported intra-assay CV is 5.9% and inter-assay CV is 8.2%. Recovery averages are 97% (range 91-103) for serum and 99% (range 93-105) for plasma. Linearity in serum (N=5) is 101-112% at 1:2, 97-107% at 1:4, 102-112% at 1:8 and 89-97% at 1:16, with parallel ranges reported for EDTA plasma and heparin plasma.

SFRP2 acts as a soluble modulator of Wnt signaling with a role in regulating cell growth and differentiation in specific cell types, and may be important for eye retinal development and for myogenesis; methylation of the gene has been described as a potential marker for the presence of colorectal cancer. The product is for research use only.