Human Secreted Frizzled Related Protein 1 (SFRP1) ELISA Kit

Quantitative immunoassay from Assay Genie for measuring human Secreted Frizzled Related Protein 1 (SFRP1), a soluble modulator of Wnt/β-catenin signaling that influences stem cell maintenance, tissue patterning, and tumor-suppressor activity.

Research applications include oncology studies where SFRP1 silencing via promoter hypermethylation has been documented across colorectal, breast, hepatocellular, and lung carcinomas, alongside investigations of renal interstitial fibrosis, cardiac remodeling after myocardial injury, bone density regulation, and osteoarthritis progression.

The kit supplies pre-coated plate wells, biotinylated detection reagent, enzyme conjugate, calibrator standards, wash concentrate, and substrate solution—enough reagents for 96 assays run as a single plate. The absorbance-based colorimetric readout integrates directly with standard laboratory plate readers, supporting high-throughput workflows.

Configured for in vitro research, the assay quantifies SFRP1 concentrations across prepared human tissue homogenate samples, cell culture supernatants, and assorted human biofluid specimens. Unopened components require refrigerated storage to preserve reagent stability through the kit's shelf life; the product is for research use only and has not been validated for diagnostic applications.