Human SCO1 ELISA Kit (HUFI08496)

Quantitative measurement of human SCO1 protein in research samples from Assay Genie supports studies of mitochondrial cytochrome c oxidase assembly and copper homeostasis. SCO1 acts as a metallochaperone that delivers copper ions to the COX2 subunit of respiratory chain complex IV, and altered expression has been documented in disorders linked to oxidative phosphorylation failure and Leigh syndrome pathology.

The kit provides pre-coated microplate strips, lyophilized standards, biotinylated detection reagent, streptavidin-HRP conjugate, wash buffer, and substrate solution, packaged in individual vials and sealed pouches. Unopened kits should be stored refrigerated until the expiration date; after first use, remaining reagents are kept refrigerated and protected from light to preserve activity.

Each microwell receives a small liquid sample, and researchers investigating mitochondrial disease pathogenesis, copper metabolism defects, or cytochrome c oxidase deficiency will find the assay useful for quantifying endogenous SCO1 in cell lysates and human-derived specimens. The procedure runs as an in vitro immunoassay suitable for laboratory research use only, with absorbance read on a conventional microplate reader. Reagents are intended for research applications and are not validated for diagnostic procedures.