Human Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (ATP2A1) ELISA Kit

Assay Genie offers a colorimetric ELISA-format kit for the quantitative measurement of Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (ATP2A1) in human samples. Compatible specimens include serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids, with reactivity confirmed for human research applications in the neurosciences.

Performance specifications reported by the manufacturer include a detection range of 0.312-20 ng/mL and a sensitivity of 0.156 ng/mL. Intra-assay precision is 5.9% CV and inter-assay precision is 7.2% CV. Recovery has been measured at 94% for serum and 96% for plasma. Specificity covers natural and recombinant human ATP2A1, also known by synonyms including SERCA1, Calcium pump 1, and Endoplasmic reticulum class 1/2 Ca(2+) ATPase. Storage is recommended at -20°C with shipping at +4°C.

ATP2A1 is a magnesium-dependent enzyme that acts as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum membrane, contributing to calcium sequestration involved in muscular excitation and contraction. Dysregulation of ATP2A1 has been linked to various muscular disorders, making it a valuable biomarker for muscle-related research.