Human SAFB2 ELISA Kit (HUFI06668)

Scaffold Attachment Factor B2 (SAFB2) is a nuclear matrix-associated protein that organizes higher-order chromatin architecture through binding to matrix attachment regions (MARs) and contributes to transcriptional repression of stress-responsive genes. The Assay Genie Human SAFB2 ELISA Kit enables quantitative measurement of this chromatin regulator in human research specimens, supporting investigations of estrogen receptor signaling, breast carcinoma biology, prostate cancer progression, and oxidative stress adaptation. Configured for 96 Assays, the kit is intended for research use only and fits laboratory workflows probing nuclear architecture, RNA polymerase II regulation, and chromatin dynamics.

Reagent contents typically include a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP conjugate, calibrator standard, wash buffer concentrate, and substrate solution for color development. End users commonly apply the assay to clinical serum samples and plasma specimens when evaluating circulating biomarker associations, and the protocol also accommodates cell lysate preparations from cultured mammalian cells. Storage recommendations generally call for refrigeration of the sealed kit at standard cold-chain temperatures, with protection of light-sensitive reagents between runs to preserve stability through the stated shelf life.

Each manufactured lot is qualified against a recombinant SAFB2 calibrator, allowing interpolation of unknown samples along a standard curve. The platform supports interrogation of SAFB1/SAFB2 heterodimer dynamics, since SAFB2 frequently partners with its paralog SAFB1 within ribonucleoprotein complexes that govern RNA polymerase II pause-site selection and splice-site recognition. Investigators studying hormone-driven tumorigenesis, oxidative stress adaptation, and chromatin remodeler crosstalk use this reagent to bridge cell-based findings with translational specimen measurements.