Human sAC (Soluble Adenylate Cyclase) ELISA Kit (HUES02639)

Assay Genie's sAC ELISA kit leverages the well-characterized bicarbonate- and Ca²⁺-regulated cyclase ADCY10, supporting quantitative studies in reproductive biology, mitochondrial metabolism, and intracellular signaling research where cyclic AMP dynamics are of interest. Typical workflows involve an overnight incubation at 2-8°C followed by colorimetric detection at 450 nm, with sample volumes of 100 µL per well loaded into the pre-coated microplate wells provided in the kit.

Researchers commonly apply the assay to characterize sAC expression in spermatozoa and capacitation studies, prostate tissue lysates, neuronal cell cultures, and metabolic disease models. For best results, serum and plasma samples should be aliquoted and stored at -20°C, with limited freeze-thaw cycles recommended to preserve epitope integrity. The standard curve typically spans a working range across several log dilutions, enabling sensitive detection of endogenous sAC levels in conditioned media, cell lysates, and tissue homogenates prepared from human research specimens, with each assay run accommodating up to 96 samples per microplate.