Human S1P2/EDG5 ELISA Kit

Designed for the quantitative measurement of human S1P2 (EDG5), this Sandwich ELISA (Double Antibody) from Assay Genie supports detection in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples using a colorimetric detection method.

The associated Uniprot identifier is O95136, and the target is also known by synonyms including S1PR2, EDG5, sphingosine 1-phosphate receptor 2, endothelial differentiation G-protein coupled receptor 5, AGR16, GPCR13, GPCR18, H218, LPB2, and EDG-5. S1P2 (EDG5) is a G protein-coupled receptor involved in cellular processes such as cell migration, proliferation, and survival, with dysregulation implicated in cancer, cardiovascular disorders, and inflammatory conditions.

The workflow involves reagent and plate preparation, primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody binding at 37°C for 60 minutes, HRP-Streptavidin binding at 37°C for 30 minutes, TMB substrate color development in the dark for 10–20 minutes, and absorbance measurement at 450 nm after adding stop solution. Included components comprise a dismountable ELISA microplate, lyophilized standard, biotin-labeled antibody (100X), HRP-Streptavidin conjugate (SABC, 100X), TMB substrate, sample dilution buffer, antibody dilution buffer, SABC dilution buffer, stop solution, wash buffer (25X), plate sealer, and a technical manual. Storage is at 2–8°C for 12 months.