Human S100A8 ELISA Kit

From Assay Genie, this sandwich ELISA, double antibody kit is designed to measure S100A8 protein in human samples. Compatible sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay uses a colorimetric detection method with TMB substrate, and absorbance is read at 450 nm.

The kit offers a sensitivity of 0.375 ng/mL and a detection range of 0.625–40 ng/mL. It should be stored at 2–8°C for 12 months. The target protein is catalogued under UniProt ID P05109. The kit is intended for research applications including cell death and the study of inflammatory conditions, where S100A8 has been associated with diseases such as rheumatoid arthritis, inflammatory bowel disease, and psoriasis.

The procedure involves equilibrating reagents, primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody incubation at 37°C for 60 minutes, HRP-Streptavidin (SABC) incubation at 37°C for 30 minutes, TMB color development in the dark for 10–20 minutes, and a stop reaction with absorbance measured at 450 nm.