Human S100 A2 PharmaGenie ELISA Kit (SBRS1050)

Quantitative measurement of the nuclear EF-hand calcium-binding protein S100A2 in research samples supports investigation of epithelial differentiation, keratinocyte regulation, and tumor-progression pathways in lung and breast tissues. This immunoassay from Assay Genie is supplied as a complete microplate kit and is intended for research use only. S100A2 is implicated in p53-mediated tumor suppression and has been examined as a biomarker candidate in non-small cell lung carcinoma and in chemosensitivity studies.

The kit is calibrated for human samples and offers reproducible standard curves across an analytical working range. Components include pre-coated wells, lyophilized standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer concentrate, substrate solution, and stop reagent, providing all reagents required for a standard curve and sample quantification run. Run-to-run precision is supported by intra-assay and inter-assay CVs documented in the enclosed certificate of analysis. Store the kit refrigerated and protected from light; consult the product insert for the exact storage temperature of opened components.

Standard dilutions allow construction of an 8-point curve for quantification, and assay setup is straightforward on a standard plate reader with absorbance readout. The kit handles cell culture supernatant, plasma, and serum matrices, with separate protocols for each. Researchers studying squamous differentiation, psoriasiform skin models, or S100-family signaling networks will find this assay well-matched to standard ELISA workflows.