Human S-Phase Kinase Associated Protein 2 (SKP2) ELISA Kit

SKP2 operates as the substrate adaptor of the SCF^Skp2 ubiquitin ligase complex, directing polyubiquitination of phosphorylated cell cycle inhibitors including p27^Kip1, p21, and cyclin E, thereby controlling progression through the G1/S checkpoint. Researchers investigating lymphomagenesis, prostate carcinoma progression, or p27 deregulation commonly measure SKP2 abundance in tumor-derived lysates and tissue homogenates to correlate expression with proliferative index and patient prognosis. Each Assay Genie microplate-based kit supplies a pre-coated ninety-six assay plate with lyophilized recombinant standards, wash concentrate, diluent buffers, chromogen substrate, and stop solution. The assay quantifies human SKP2 across a working range of 0.156–10 ng/mL, requires 50 µL of sample per well, and achieves a minimum detectable concentration of approximately 0.08 ng/mL. Store unopened components refrigerated at 4°C; reconstituted reagents should be aliquoted and frozen at -20°C to preserve activity across repeated freeze-thaw cycles. Intended for research use only.