Human RTN1 (Isoform RTN1-C) ELISA Kit (HUFI06690)

Quantitative measurement of the RTN1-C isoform in human samples is supported by this isoform-selective immunoassay kit from Assay Genie. RTN1 (Reticulon-1) belongs to the reticulon family of endoplasmic reticulum-resident membrane proteins and has been investigated in neuroendocrine biology, neuroaxonal injury biomarker discovery, and tumor research, with published work linking RTN1-C expression to glioma progression and colorectal carcinoma studies.

The assay uses a matched pair of monoclonal antibodies directed against the RTN1-C-specific region, allowing isoform-resolved detection rather than total reticulon signal. Researchers commonly combine this kit with companion RTN1-A and RTN1-B isoform assays when profiling isoform-specific expression patterns in cell culture lysates, frozen tissue homogenates, or conditioned media from in vitro experiments.

Each kit supplies a pre-coated microplate, lyophilized recombinant standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer concentrate, TMB substrate, and stop reagent, sufficient for one full plate run. Reconstituted standards should be aliquoted and frozen immediately; avoid repeated freeze-thaw cycles to preserve the calibration curve. Bring all reagents to room temperature before use and protect the substrate from light. The kit is intended for research use only and is not validated for diagnostic procedures.