Human RtcB ELISA Kit (HUFI06705)

This colorimetric kit from Assay Genie provides quantitative measurement of human RtcB (also known as HSPC117 or C5orf21), an RNA ligase that joins 2',3'-cyclic phosphate ends to 5'-OH ends during tRNA splicing and other RNA repair pathways. The capture antibody is specific to the human ortholog, and the kit is intended for research use only, not for diagnostic procedures. Each kit contains a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP conjugate, calibrated standards, wash buffer, and substrate sufficient for one full plate of determinations. Reagents should be stored refrigerated upon receipt, with lyophilized standards reconstituted and aliquoted to minimize freeze-thaw cycles. Detection is performed using a standard microplate reader following the in vitro protocol, with typical runs completed within several hours. Sample preparation is straightforward: collected fluid samples are added directly to wells without prior extraction or purification steps.