Human RRP1 ELISA Kit (HUFI06702)

The Human RRP1 ELISA Kit from Assay Genie quantifies Ribosomal RNA Processing 1 (RRP1), a conserved nucleolar protein that participates in ribosomal RNA maturation and 60S subunit assembly. RRP1 expression is being investigated in cancer biology, ribosome biogenesis disorders, and cell proliferation studies, where researchers use serum and plasma matrices to explore its potential as a biomarker of nucleolar activity.

This sandwich-format immunoassay ships as a complete 96 Assays package containing a pre-coated microplate, lyophilized recombinant RRP1 standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash concentrate, TMB substrate, and stop solution. The protocol uses a 100 µL sample volume per well, with standards and unknowns typically run in duplicate to generate a standard curve. Unused reconstituted standards and detection reagents should be refrigerated upon receipt to preserve activity, and the kit is intended for research use only.

Common research applications include profiling RRP1 levels across tumor and matched-normal tissue cohorts, comparing expression in proliferating versus quiescent cell lines, and supporting mechanistic studies on ribosome biogenesis under stress conditions such as nutrient deprivation or chemotherapeutic exposure.