Human RRAS2 ELISA Kit (HUFI06718)

RRAS2 (Ras-Related 2), also referenced in the literature as TC21, is a small GTP-binding protein belonging to the Ras superfamily of guanine nucleotide-binding proteins. It transmits signals from activated membrane receptors to downstream effectors that govern cytoskeletal reorganization, integrin-mediated adhesion, migration, and proliferative responses. By providing quantitative measurement of human RRAS2 protein, this Assay Genie kit supports laboratory studies of oncogenic Ras pathway dysregulation, Ras-MAPK-related developmental syndromes, and mechanistic investigations of small GTPase signaling crosstalk.

The kit is configured as a complete microplate-format set of reagents, including pre-coated capture wells, lyophilized calibrator standards, biotinylated detection reagent, streptavidin-enzyme conjugate, and substrate solution. All reagents are supplied in single-use vials to limit freeze-thaw cycles, and unopened components should be stored refrigerated and protected from light until first use. Reconstituted standards and working-strength detection reagents should be aliquoted and held frozen for subsequent experimental runs within the kit's stated shelf life.

Compatible specimen preparations include clarified cell lysates and conditioned cell culture media, with appropriate dilution recommended before loading. Each plate incorporates a multi-point standard curve used to interpolate sample RRAS2 concentrations across the working range of the assay, supporting consistent comparison across biological replicates and treatment conditions.