Human RPF1 (Ribosome production factor 1) ELISA KIT (HUFI08236)

Ribosome biogenesis research requires sensitive quantitative tools for measuring individual assembly factors, and the Human RPF1 ELISA Kit from Assay Genie provides a complete in vitro immunoassay for ribosome production factor 1 in human specimens. RPF1 is a conserved nucleolar protein required for processing of 27S pre-rRNA and for maturation of the 60S ribosomal subunit, making it central to ribosome assembly and overall protein synthesis capacity. Recent literature has linked altered RPF1 expression to proliferation, migration, and invasion phenotypes in colorectal and gastric cancer models, positioning the protein as a candidate biomarker in translational oncology.

The kit arrives as a complete package containing a pre-coated microplate, lyophilized recombinant standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer concentrate, TMB substrate, and stop reagent. All components are supplied in individual vials within a kit box and remain stable when stored refrigerated; aliquoting reconstituted standards avoids repeated freeze-thaw cycles and protects assay performance. Each kit provides enough material for 96 individual determinations, accommodating both small pilot studies and larger cohort screens.

Investigators studying ribosome assembly pathways, the molecular drivers of tumor cell growth, or mechanisms of protein synthesis regulation can apply this research use only assay to compare RPF1 abundance across experimental conditions and patient-derived samples. The format supports quantitative comparison between treated and control groups in cell culture workflows and in tissue-derived lysates from translational study cohorts.