Human RPAP3 ELISA Kit (HUFI06767)

The RPAP3 protein (RNA Polymerase II Associated Protein 3) is a core component of the R2TP co-chaperone complex, which facilitates the assembly of RNA polymerase II and small nucleolar ribonucleoproteins. This Assay Genie kit provides a plate-based immunoassay to quantify endogenous RPAP3 levels in human clinical specimens, supporting studies in transcription regulation, cancer biology, and RNA-processing pathways. Each kit contains a pre-coated microplate, detection antibodies, calibration standards, and assay buffers sufficient for 96 individual reactions run in a standard well-plate format. Researchers apply the assay to investigate altered R2TP complex activity in tumor-derived samples and to correlate RPAP3 expression with polymerase II assembly defects. Reagent volumes are calibrated for 100 µL sample and standard additions per well, with chromogenic signal development measured on a standard plate reader. Store unopened kits refrigerated and protect from light, and avoid repeated freeze-thaw cycles of reconstituted standards to preserve antibody binding activity. The kit is intended for research use only and is not validated for diagnostic procedures.