Human RPAP2 ELISA Kit (HUFI06766)

RPAP2 (RNA Polymerase II-Associated Protein 2), also known as RTR1, functions as a phosphatase that dephosphorylates Ser5 residues on the RNA Polymerase II C-terminal domain, positioning it as a key regulator of transcription elongation, chromatin remodeling, and gene expression programs. Quantitative analysis of this target in human biofluid specimens is widely applied in cancer biology research, mechanistic studies of transcriptional dysregulation, and biomarker discovery workflows, including investigations of how RPAP2 expression changes correlate with tumor progression and therapeutic response.

The Assay Genie workflow involves loading 100 uL of prepared sample per microplate well alongside a serial dilution of recombinant reference standard, followed by chromogenic substrate development and absorbance reading. Reagent components should be stored at -20 degrees C prior to first use, with the sealed plate held at 4 degrees C and reconstituted working standards aliquoted to limit repeated freeze-thaw cycles. The complete package provides calibrated assay and wash buffers, biotinylated detection antibody, streptavidin-HRP conjugate, chromogen solution, and a pre-coated plate sufficient for running standard curves and unknown specimens in parallel. This ELISA is configured for research use only and is not intended for clinical diagnostic procedures.