Human RPA3 ELISA Kit (HUFI06763)

This Sandwich ELISA Kit from Assay Genie quantitatively measures human Replication Protein A3 (RPA3), the 14 kDa third subunit of the heterotrimeric single-strand DNA-binding RPA complex that participates in DNA replication, nucleotide excision repair, and homologous recombination. The 96 Assays microplate format is calibrated for secreted RPA3 detection in serum and plasma samples, supporting translational research on DNA damage response, chemoresistance mechanisms, and tumor biomarker validation. The protocol requires a 100 uL sample volume per well in a standard in vitro workflow, with human-specific capture and detection antibodies showing no significant cross-reactivity with rat or mouse RPA3. Unopened kits should be refrigerated at 2-8 degrees C, with the streptavidin-HRP conjugate protected from light and the standard protein aliquoted to limit freeze-thaw cycles across experiments. Intended for research use only, the assay provides a reproducible quantitative tool for comparing RPA3 levels between patient cohorts and control populations in genome stability studies.