Human Roundabout homolog 4 (ROBO4) ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA Kit enables quantitative detection of human Roundabout homolog 4 (ROBO4) using a colorimetric method. The assay is validated for human reactivity and accepts serum, plasma, tissue homogenates, cell culture supernates and other biological fluids as sample inputs, with specificity directed at natural and recombinant human ROBO4 (UniProt Q8WZ75).

Performance figures reported for the kit include a detection range of 0.156-10 ng/mL and a sensitivity of 0.1 ng/mL. Precision is given as an intra-assay CV of 4.1% and inter-assay CV of 7.8%. Spike-and-recovery testing returns an average of 103% (range 97-109%) in serum and 105% (range 99-111%) in plasma, with linearity assessed across 1:2 to 1:16 dilutions in serum, EDTA plasma and heparin plasma matrices.

ROBO4 is a transmembrane receptor best known as a receptor for Slit proteins, including SLIT2, where it contributes to angiogenesis and vascular patterning and may mediate inhibition of primary endothelial cell migration. Dysfunction of ROBO4 has been linked to cancer, cardiovascular disorders and inflammatory conditions, supporting its use as a biomarker in cancer, angiogenesis, vascular biology and related disease pathway research. The kit is supplied for research use only.