Human RNMTL1 ELISA Kit (HUFI06777)

The Human RNMTL1 ELISA Kit from Assay Genie is intended for research use only and supports quantitative measurement of RNA methyltransferase-like 1, a mitochondrial enzyme that performs 2'-O-methylation of the mitochondrial 16S rRNA subunit. The assay is useful for investigations of mitochondrial ribosome assembly, oxidative phosphorylation, and cellular respiration pathways, with applications in studies of neurodegeneration, metabolic disease, and mitochondrial disorders.

Each well uses 100 µL of sample, and the microplate arrives pre-coated with capture antibody. Researchers add standards and unknown samples, perform the incubation and wash steps, then add detection antibody and substrate. Absorbance readings are interpolated against a standard curve generated from serial dilutions of the supplied reference material to determine RNMTL1 concentrations in test specimens.

Unopened kit components should be stored at 4°C and protected from light. Reconstituted standards and diluted working solutions should be aliquoted and stored frozen; limit freeze-thaw cycles to preserve activity. All reagents are validated for in vitro research use, supporting quantitative studies of mitochondrial RNA modification.